Table 1

Knockdown of Ppp2r2a reduced PPP2R2A and AKT protein levels and insulin-stimulated AKT phosphorylation

Cell lineRelative protein levels PPP2R2A (%)P valueRelative protein levels AKT (%)P valuePhosphorylation normalized to tubulin P value saline vs. insulinP value control saline vs. salineDirection of effect on basal phosphorylationP value control insulin vs. insulinDirection for stimulation
SalineInsulin
ControlHepa1-6100 ± 24100 ± 24.5pAKT(Thr-308)2.31 ± 0.7610.78 ± 1.3<0.0001
siRNA117.55 ± 3.9<0.000142.68 ± 10.6<0.00013.51 ± 0.516.44 ± 1.780.0030.0090.0007
siRNA317.8 ± 5.6<0.000134.90 ± 12.3<0.00014.35 ± 0.514.84 ± 0.49ns0.0002<0.0001
ControlHepa1-6pAKT(Ser-473)7.23 ± 0.8927.74 ± 3.49<0.0001
siRNA14.83 ± 1.3517.31 ± 4.70<0.00010.0040.001
siRNA38.73 ± 1.0417.81 ± 1.94<0.00010.020.0001
ControlC2C12100 ± 37.5100 ± 30.3pAKT(Thr-308)1.19 ± 0.258.26 ± 2.58<0.0001
siRNA127.33 ± 6.7<0.000182.19 ± 6.80.064.45 ± 0.636.03 ± 1.310.02<0.0001ns
siRNA335.78 ± 20.3<0.000173.22 ± 18.30.014.10 ± 0.875.06 ± 2.09ns<0.00010.03
ControlC2C12pAKT(Ser-473)5.06 ± 2.1136.83 ± 11.8<0.0001
siRNA17.54 ± 1.4425.40 ± 2.94<0.00010.030.04
siRNA38.25 ± 0.8719.24 ± 6.990.0030.0060.01
Control3T3-L1100 ± 18.4104 ± 14.6pAKT(Thr-308)0.53 ± 0.191.98 ± 0.26<0.0001
siRNA164.63 ± 17.3<0.000177.08 ± 12.3<0.00011.00 ± 0.121.62 ± 0.91ns0.0006ns
siRNA365.26 ± 11.4<0.000172.31 ± 8.1<0.00010.79 ± 0.271.50 ± 0.86nsnsns
Control3T3-L1pAKT(Ser-473)0.61 ± 0.032.45 ± 0.39<0.0001
siRNA11.99 ± 0.512.45 ± 0.38ns<0.0001ns
siRNA31.10 ± 0.222.25 ± 0.480.00030.0003ns
  • ns, not significant; p, phosphorylation.