Table 2

Knockdown of Ppp2r2a reduced downstream insulin AKT signaling

Cell lineRelative protein levels GSK-3βP valueRelative protein levels p70S6KP value% PhosphorylationP value saline vs. insulinP value control saline vs. salineDirection of effect on basal phosphorylationP value control insulin vs. insulinDirection for stimulation
SalineInsulin
ControlHepa1-699 ± 6.8pGSK-3β53.03 ± 7.12107 ± 10.80<0.0001
siRNA1113.92 ± 12.9ns65.27 ± 7.7961.95 ± 9.16ns0.01<0.0001
siRNA3109.13 ± 13.6ns69.68 ± 3.2958.09 ± 5.52ns0.0004<0.0001
ControlHepa1-6100 ± 5.5pp70S6K4.24 ± 0.3510.22 ± 0.73<0.0001
siRNA1109.13 ± 13.6ns10.58 ± 1.1910.62 ± 1.34ns<0.0001ns
siRNA390.61 ± 17.6ns9.44 ± 1.7910.49 ± 1.74ns<0.0001ns
ControlC2C12100 ± 12.45pGSK-3β59.16 ± 15.65105.54 ± 11.2<0.0001
siRNA1113.94 ± 8.90.0445.76 ± 3.1656.16 ± 19.59nsns0.0003
siRNA3151.30 ± 32.90.0258.45 ± 14.0369.13 ± 10.40nsns0.0001
ControlC2C12100 ± 20.5pp70S6K6.37 ± 2.0712.36 ± 2.330.0008
siRNA1129.41 ± 16.20.029.71 ± 0.7010.51 ± 0.510.040.003ns
siRNA399.99 ± 6.90.0110.76 ± 0.859.92 ± 1.30ns0.0007ns
Control3T3-L1100 ± 29.9pGSK-3β44.47 ± 5.7589.48 ± 2.90<0.0001
siRNA1101.15 ± 33.7ns72.44 ± 7.4092.36 ± 10.890.004<0.0001ns
siRNA3105.03 ± 27.9ns65.9 ± 6.2486.16 ± 13.430.0070.0001ns
Control3T3-L199 ± 19.39pp70S6K4.74 ± 0.818.23 ± 0.42<0.0001
siRNA1105.39 ± 28.5ns4.91 ± 0.825.06 ± 0.41nsns<0.0001
siRNA399.99 ± 6.9ns6.68 ± 0.717.85 ± 0.6380.010.001ns
  • ns, not significant; p, phosphorylation.