TABLE 2

Effect of glucose on total lipase activity in homogenates of INS-1 cells

Time (h)3.3 mmol/l glucose (mU/mg protein)25 mmol/l glucose (mU/mg protein)% Induction
44.00 ± 0.134.10 ± 0.05103
83.60 ± 0.083.93 ± 0.12109
163.94 ± 0.104.90 ± 0.08***125
323.68 ± 0.115.62 ± 0.06***153
  • Homogenates of INS-1 cells were assayed for diglyceride lipase activity. One unit of enzyme activity corresponds to 1 μmol of fatty acid released per minute at 37°C. The values in the table are means ± SE from one experiment with quadruplicates of each condition. The HSL activity was analyzed in triplicate.

  • ***

    *** P < 0.001.