TABLE 1

Regulation gene expression in leptin-deficient mice

Unigene clusterFunctionsGene nameExpression ratio
ob/ob:wild-type
Upregulated in  ob/ob mice
 Mm.4533Lipoprotein metabolismApo A-IV10.3 (6.1–14.5)
 Mm.20697EST7.3 (4.0–10.6)
 Mm.23223EST5.4 (−0.9–11.7)
 Mm.1620Phospholipid binding proteinAnnexin V4.0 (3.1–4.9)
 Mm.27136EST highly similar to IIP23.7 (2.5–4.9)
 Mm.22966EST3.6 (0.6–6.6)
 Mm.31741EST moderately similar to glyceraldehyde 3-phosphate dehydrogenase3.3 (2.8–3.8)
 Mm.23601EST3.2 (0.9–5.6)
 Mm.3444Signaling moleculeBromodomain-containing 23.1 (2.7–3.4)
 Mm.32353EST3.0 (0.7–5.4)
 Mm.1620Phospholipid binding proteinAnnexin V3.0 (2.8–3.2)
 Mm.14022Respiratory chainCytochrome C oxidase, subunit VIIIa3.0 (1.4–4.5)
 Mm.30567EST2.5 (2.0–3.0)
 Mm.20407EST weakly similar to pancreatic lipase–related protein 12.5 (1.6–3.3)
 Mm.7524Cytoskeletal proteinDystroglycan 12.5 (1.2–3.7)
 Mm.300Carbonic anhydrase 32.4 (2.1–2.6)
 Mm.3760Fatty acid metabolismFatty acid synthase2.4 (1.1–3.8)
 Mm.27395EST weakly similar to glutathione S-transferase2.3 (1.6–3.1)
 Mm.43415Respiratory chainCytochrome C oxidase, subunit VI a, polypeptide 12.3 (1.4–3.2)
 Mm.1779Fatty acid metabolismSterol carrier protein 22.3 (2.1–2.4)
 Mm.28882EST moderately similar to elongation factor 1-α2.3 (1.8–2.7)
 Mm.2329Hemoglobin β-pseudogene bh32.2 (1.4–3.0)
 Mm.21571EST2.2 (1.6–2.7)
 Mm.14796Oxidative stressGlutathione S-transferase2.1 (1.6–2.6)
 Mm.2475Glutaryl-CoA dehydrogenase2.1 (1.4–2.8)
 Mm.16763GlycolysisFructose-bisphosphate aldolase A2.1 (1.4–2.8)
 Mm.2607Stress proteinHeat-responsive protein-122.1 (2.0–2.2)
 Mm.32261EST2.1 (1.3–2.8)
 Mm.10633Ketone body productionHMG-CoA synthase 22.0 (1.8–2.3)
 Mm.2607Stress proteinHeat-responsive protein-122.0 (1.9–2.2)
 Mm.1703Cytoskeletal proteinTubulin-β 52.0 (1.8–2.2)
 Mm.1155Cytoskeletal proteinTubulin-α 4 chain2.0 (1.5–2.5)
 Mm.32254EST2.0 (1.0–3.0)
 Mm.29057EST highly similar to myosin regulatory light chain 2-A2.0 (1.5–2.5)
 Mm.8911Protein processingProteasome-β-type subunit 52.0 (1.5–2.4)
 Mm.22941EST2.0 (1.0–2.9)
 Mm.2648Signaling moleculeCalmodulin1.9 (1.8–2.1)
 Mm.2491Fatty acid metabolism3-Hydroxyacyl-CoA dehydrogenase1.9 (1.3–2.5)
 Mm.5121Peptide metabolismPeptidylglycine α-amidating monooxygenase1.9 (1.2–2.7)
 Mm.19094Respiratory chainCytochrome C oxidase, subunit VI a, polypeptide 11.9 (1.4–2.4)
 Mm.27677Respiratory chainEST highly similar to NADH-ubiquinone oxidoreductase 13Kd-b subunit1.9 (1.5–2.3)
 Mm.28683EST1.9 (1.4–2.3)
 Mm.12556Mitochondrial metabolism relatedUncoupling protein 21.8 (0.8–2.9)
 Mm.1025Transcription factorNFED 21.8 (1.4–2.3)
 Mm.29240EST1.8 (1.0–2.7)
 Mm.3164Respiratory chainCytochrome P450 2D91.8 (1.6–2.1)
 Mm.7500Ferritin light chain 11.8 (1.4–2.2)
 Mm.30250EST highly similar to antiquitin1.8 (1.7–1.9)
 Mm.29842Respiratory chainEST highly similar to NADH-ubiquinone oxidoreductase 51Kd subunit1.8 (1.6–2.0)
 Mm.23448EST1.8 (1.6–2.0)
 Mm.3476Signaling moleculeCatenin-β1.8 (1.1–2.5)
 Mm.9703Copper transport proteinAtox11.8 (1.3–2.2)
 Mm.31571EST1.8 (1.6–2.0)
 Mm.2585Brain protein I31.8 (1.1–2.4)
 Mm.37656EST moderately similar to proteasome Z subunit precursor1.8 (1.5–2.0)
 Mm.35081EST weakly similar to titin1.7 (1.2–2.3)
 Mm.424Respiratory chainATPase, Na+/K+ β3 polypeptide1.7 (1.4–2.1)
 Mm.22668Ketone body productionHMG-CoA lyase (mitochondrial)1.7 (1.3–2.1)
 Mm.32259EST1.7 (1.3–2.1)
 Mm.26993Respiratory chainCytochrome P450 3A251.7 (1.4–2.0)
 Mm.30806Protein synthesis60S Ribosomal protein L191.7 (1.4–2.0)
 Mm.24314EST1.7 (1.5–1.8)
 Mm.31336EST1.7 (1.4–1.9)
 Mm.4266Integral membrane protein 2 B1.6 (1.5–1.8)
 Mm.37097EST1.6 (1.2–2.1)
 Mm.29821Peroxiredoxin 31.6 (1.4–1.9)
 Mm.29628Oxidative stressEST weakly similar to acetylglucosaminyltransferase-like protein1.6 (1.3–1.9)
 Mm.26949EST highly similar to electron transfer flavoprotein α-subunit1.6 (1.3–1.9)
 Mm.2576BP 44-like Protein1.6 (1.2–2.0)
 Mm.13886HelicaseSui11.6 (1.3–1.9)
 Mm.1970Carbohydrate metabolism/ signaling proteinProtein phosphatase 1, catalytic subunit, α isoform1.6 (1.2–2.0)
 Mm.34470EST1.6 (1.2–2.0)
 Mm.32251EST1.6 (1.3–1.9)
 Mm.29633EST1.6 (1.3–1.8)
 Mm.13886Sui11.6 (1.4–1.7)
 Mm.34775Carbohydrate metabolismPyruvate dehydrogenase Elα subunit1.6 (1.4–1.8)
 Mm.426Glutathione metabolismGlutathione S-transferase, pi 21.6 (1.3–1.8)
 Mm.22485EST weakly similar yeast D-lactate dehydrogenase1.5 (1.4–1.7)
 Mm.2050EST highly similar to ribosomal protein L151.5 (1.3–1.7)
 Mm.26272EST1.5 (1.3–1.8)
 Mm.4859EST highly similar to Rer1 protein1.5 (1.4–1.7)
 Mm.29677EST1.5 (1.4–1.7)
 Mm.2491Fatty acid metabolism3-hydroxyacyl CoA dehydrogenase1.5 (1.2–1.8)
 Mm.30012Lipoprotein metabolismHDL-binding protein1.5 (1.4–1.6)
 Mm.9925Carbohydrate metabolismNADP-dependent isocitrate dehydrogenase1.5 (1.4–1.6)
 Mm.32222EST1.5 (1.4–1.6)
 Mm.42472Microsomal modifying enzymeUDP-glucuronosyltransferase 1 family member 11.5 (1.4–1.6)
 Mm.4299Fatty acid metabolismMethylmalonyl CoA mutase1.5 (1.3–1.6)
 Mm.1486ADP ribosylation factor 4ADP ribosylation factor 41.4 (1.4–1.5)
 Mm.46028EST highly similar to CGI-26 protein1.4 (1.4–1.4)
 Mm.21209EST1.4 (1.3–1.5)
 Mm.28492EST highly similar to KIAA0652 protein1.4 (1.3–1.5)
 Mm.23165EST weakly similar to ankyrin repeat-containing protein1.4 (1.3–1.5)
Downregulated in ob/ob mice
 Mm.29973Respiratory ChainCytochrome P450, 2c400.30 (0.25–0.35)
 Mm.141936Serum ProteinIGF-binding protein 20.35 (0.28–0.42)
 Mm.32801EST0.47 (0.40–0.55)
 Mm.14455Signaling proteinTransforming growth factor, β induced, 68 kDa0.54 (0.48–0.59)
 Mm.2632Transcription factorLatexin0.55 (0.47–0.62)
 Mm.3288Transcription factorT-lymphocyte regulatory protein 10.55 (0.44–0.66)
 Mm.28502RNA metabolismDouble-stranded RNA-specific adenosine deaminase0.56 (0.30–0.81)
 Mm.32824EST0.57 (0.41–0.73)
 Mm.955Protein traffickingμ-chain association protein0.58 (0.45–0.70)
 Mm.32225EST0.58 (0.41–0.75)
 Mm.29014Signaling proteinRAS-related C3 botulinum substrate 10.58 (0.38–0.78)
 Mm.30694EST0.58 (0.39–0.78)
 Mm.22173Cell adhesionSelectin, platelet (p-selectin) ligand0.59 (0.53–0.65)
 Mm.19131Immune responseComplement C30.60 (0.50–0.69)
 Mm.142Signaling proteinLymphocyte protein tyrosine kinase0.60 (0.37–0.83)
 Mm.4559Glutathione metabolismγ-glutamyl transpeptidase0.61 (0.45–0.77)
 Mm.16716Lysosomal proteinLysosomal membrane glycoprotein 10.61 (0.52–0.69)
 Mm.3420Transcription factorNF-κB10.61 (0.48–0.75)
 Mm.32829EST0.61 (0.46–0.77)
 Mm.833Protein traffickingClathrin coat assembly protein AP190.61 (0.48–0.75)
 Mm.29524Membrane channelNuclear chloride channel protein0.61 (0.46–0.76)
 Mm.23936EST0.61 (0.57–0.66)
 Mm.30603EST0.63 (0.52–0.74)
 Mm.13036EST weakly similar to TSC5010.63 (0.50–0.77)
 Mm.6407EST0.63 (0.49–0.78)
 Mm.24263EST0.64 (0.56–0.71)
 Mm.542RNA metabolismRNA-binding motif protein 40.64 (0.57–0.71)
 Mm.20874Protein processingPeptidylarginine deaminase type IV0.64 (0.53–0.76)
 Mm.20964Signaling proteinGuanine nucleotide binding protein-α o0.65 (0.60–0.70)
 Mm.32274EST0.65 (0.54–0.76)
 Mm.22963EST highly similar to TEB4 protein0.65 (0.59–0.72)
 Mm.42321EST0.65 (0.56–0.74)
 Mm.32266EST0.65 (0.53–0.78)
 Mm.32472EST0.66 (0.61–0.71)
 Mm.32447EST0.66 (0.52–0.79)
 Mm.31837EST0.66 (0.58–0.74)
 Mm.11946EST weakly similar to a thyroid hormone responsive gene0.66 (0.56–0.76)
 Mm.23037EST0.66 (0.54–0.78)
 Mm.22540EST0.67 (0.62–0.70)
 Mm.13835EST weakly similar to p190-B0.67 (0.56–0.77)
 Mm.32303EST0.67 (0.51–0.82)
 Mm.32344EST0.67 (0.66–0.69)
 Mm.2453HormoneSomatostatin0.67 (0.63–0.72)
 Mm.24004EST0.67 (0.59–0.76)
 Mm.34298EST0.68 (0.62–0.73)
 Mm.21049Amino acid metabolismLysyl oxidase-20.68 (0.56–0.80)
 Mm.32795EST0.69 (0.67–0.69)
 Mm.28889EST0.69 (0.65–0.72)
 Mm.35774EST weakly similar to MAP kinase phosphatase-10.69 (0.63–0.75)
 Mm.27573EST0.69 (0.57–0.81)
 Mm.35134Calcium transportCa++ transporting ATPase0.69 (0.57–0.81)
 Mm.142Signaling proteinLymphocyte protein tyrosine kinase0.69 (0.63–0.76)
 Mm.3130Cytoskeletal proteinKinesin heavy chain member 40.69 (0.64–0.75)
 Mm.23038EST0.70 (0.65–0.74)
 Mm.29381EST weakly similar to centromeric protein E0.70 (0.66–0.74)
 Mm.32121EST0.70 (0.68–0.73)
 Mm.32421EST0.71 (0.65–0.76)
 Mm.30743EST0.71 (0.68–0.74)
 Mm.32440EST0.71 (0.69–0.74)
 Mm.42704EST0.71 (0.67–0.76)
 Mm.15105Signaling proteinInositol polyphosphate-5-phosphatase, 145 kDa0.72 (0.68–0.75)
 Mm.54517EST highly similar to nuclear pore complex protein NUP155.0.72 (0.66–0.78)
 Mm.45985MetalloproteaseBone morphogenetic protein 10.72 (0.67–0.77)
 Mm.23007EST0.72 (0.72–0.73)
 Mm.1685Signaling proteinPlexin 30.72 (0.69–0.76)
 Mm.6977Protein processingTrypsin 40.72 (0.68–0.77)
 Mm.32515EST0.73 (0.70–0.76)
 Mm.22789EST weakly similar to retinal-cadherin0.73 (0.73–0.74)
 Mm.28854EST weakly similar to type VI collagen-α 3 subunit0.74 (0.72–0.77)
 Mm.22818EST0.75 (0.73–0.76)
 Mm.32162EST0.75 (0.73–0.77)
  • EST, expressed sequence tag.