TABLE 2

Regulation of genes

Unigene clusterGene nameExpression ratioExpression ratio
(ob/ob vehicle)
(ob/ob)
(ob/ob leptin)(wild-type)
Regulated by leptin treatment in ob/ob mice
 Mm.20697EST7.3 (4.3–10.2)7.44
 Mm.23889EST4.2 (2.4–6.0)No change
 Mm.4533Apol A-IV3.9 (2.6–5.2)10.3
 Mm.16763Fructose-bisphosphate aldolase a3.1 (1.7–4.5)2.34
 Mm.31741EST moderately similar to Glyceraldehyde 3-phosphate dehydrogenase2.7 (1.7–3.8)3.39
 Mm.23223EST2.6 (1.7–3.4)5.28
 Mm.27136EST highly similar to IIP22.3 (0.7–3.9)3.59
 Mm.41853Deoxyribonuclease II2.1 (1.4–2.9)No change
 Mm.18213Tubulin 5 β2.1 (1.6–2.7)1.83
 Mm.24148EST2.1 (1.5–2.7)No change
 Mm.1620Annexin V2.1 (1.4–2.8)2.91
 Mm.28100L-specific multifunctional β-oxidation protein2.0 (1.5–2.6)2.09
 Mm.24742Pantothenate kinase 1-β1.8 (1.2–2.4)1.75
 Mm.18549EST weakly similar to testatin1.7 (1.5–1.9)No change
 Mm.20288EST highly similar to glutathione reductase1.7 (1.4–2.0)No change
 Mm.20925Guanine nucleotide regulatory protein1.7 (1.4–2.0)No change
 Mm.140158Cytochrome P450, 511.6 (1.2–2.0)No change
 Mm.42795IL-4–derived transcript1.5 (1.4–1.6)No change
 Mm.32101EST1.5 (1.3–1.6)No change
 Mm.11816EST1.4 (1.3–1.5)1.56
 Mm.8903Branched chain-α ketoacid dehydrogenase kinase1.4 (1.3–1.5)No change
 Mm.14099Hematopoietic zinc finger protein1.3 (1.3–1.4)No change
 Mm.23304TMEFF20.75 (0.74–0.77)No change
 Mm.34527EST0.7 (0.7–0.8)No change
 Mm.27539EST0.7 (0.6–0.8)No change
 Mm.29068EST0.6 (0.5–0.8)No change
 Mm.23443EST0.6 (0.4–0.9)No change
 Mm.3040EST0.6 (0.5–0.7)No change
 Mm.23252EST0.5 (0.2–0.8)No change
Regulated by leptin independent of food intake
 Mm.30479EST3.60 (1.38–5.83)
 Mm.23581EST3.57 (0–7.49)
 Mm.2041Metallothionein 12.67 (0.03–5.31)
 Mm.4533Apo A-IV2.57 (1.51–3.63)
 Mm.34446Cyclin-dependent kinase inhibitor 1A-p212.21 (1.76–2.67)
 Mm.2585Brain protein I31.97 (1.69–2.24)
 Mm.27136EST highly similar to IIP21.96 (1.14–2.78)
 Mm.27506EST1.91 (1.63–2.19)
 Mm.27138EST1.73 (0.82–2.64)
 Mm.23411EST1.72 (1.59–1.85)
 Mm.31741EST moderately similar to glyceraldehyde 3-phosphate dehydrogenase1.71 (1.23–2.18)
 Mm.29842NADH dehydrogenase flavoprotein 11.70 (1.63–1.77)
 Mm.44501EST highly similar to gastrula zinc finger protein1.69 (1.14–2.25)
 Mm.5238Guanine nucleotide-binding protein α-13 subunit1.67 (0.58–2.77)
 Mm.26709EST1.67 (1.56–1.79)
 Mm.17533EST highly similar to BAP2-β1.62 (1.29–1.95)
 Mm.2605Retino-binding protein 41.60 (1.46–1.73)
 Mm.10633HMG-CoA1.60 (1.49–1.71)
 Mm.34742EST highly similar to citrin1.56 (1.18–1.95)
 Mm.29451EST weakly similar to B0414.81.55 (1.48–1.62)
 Mm.24014EST1.55 (1.17–1.93)
 Mm.29882EST1.54 (1.36–1.71)
 Mm.33729EST1.51 (1.20–1.83)
 Mm.28882EST moderately similar to elongation factor 1-α1.51 (1.36–1.66)
 Mm.4266Integral membrane protein 2B1.51 (1.23–1.79)
 Mm.20354Kinesin light chain 11.47 (1.30–1.64)
 Mm.31810EST1.46 (1.22–1.71)
 Mm.21761EST1.46 (1.10–1.81)
 Mm.39099EST highly similar to zinc finger protein 911.46 (1.41–1.50)
 Mm.22570Histone H1.11.45 (1.39–1.52)
 Mm.29682EST1.43 (1.28–1.58)
 Mm.13886Sui11.41 (1.38–1.43)
 Mm.23325EST1.39 (1.34–1.43)
 Mm.18443GLUT21.37 (1.31–1.42)
 Mm.5750EST0.70 (0.64–0.76)