TABLE 1

Detection of T-cell responses

AssayResult
Laboratory no. 1
 ProliferationResponses to GAD(524-543) and insulin (9-23) detected (SIs ∼10)
Laboratory no. 2
 ProliferationNo responses detected
Laboratory no. 3
 ProliferationNo responses to GAD(524-543) or insulin(9-23) Responses detected to GAD(217-236) (average SI ∼3)
 ELISA analysis of cytokines in culture supernatantsResponses detected to GAD(524-543) and insulin(9-23)(∼5-fold over background) and to GAD(217-236) (∼43-fold over background)
Laboratory no. 4
 ProliferationNo responses detected
Laboratory no. 5
 ProliferationNo responses detected
 Preexpansion of cells followed by ELISPOTResponses detected to GAD(524-543) (>100 spots over background) but not to insulin(9-23)
Laboratory no. 6
 ProliferationNo responses detected
Laboratory no. 7
 ProliferationNo responses detected
Laboratory no. 8
 ProliferationNo responses detected
Laboratory no. 10
 ProliferationNo responses detected
 ELISPOTNo responses detected
Laboratory no. 12
 ProliferationNo responses detected
 CFSE-FACSNo responses detected
 ELISPOTNo responses detected
Laboratory no. 13
 ProliferationNo responses detected
  • FACS, –fluorescence activated cell sorter.