TABLE 1

[13C]leucine enrichments in tissue fluid and bound in mitochondrial and sarcoplasmic proteins in each tissue

TissueTissue fluid (mole % excess)Mitochondrial protein (atom % excess)Sarcoplasmic protein (atom % excess)
Liver
 Saline4.95 ± 0.370.132 ± 0.0100.079 ± 0.015
 Insulin5.27 ± 0.360.102 ± 0.0140.091 ± 0.011
 INS+AA3.91 ± 0.26*0.070 ± 0.007*0.054 ± 0.008
Heart
 Saline3.98 ± 0.280.081 ± 0.0040.085 ± 0.005
 Insulin4.50 ± 0.24*0.094 ± 0.003*0.105 ± 0.004*
 INS+AA2.88 ± 0.040.068 ± 0.003*0.079 ± 0.004
Muscle
 Saline2.96 ± 0.170.020 ± 0.0030.011 ± 0.002
 Insulin2.63 ± 0.230.036 ± 0.005*0.017 ± 0.004
 INS+AA2.22 ± 0.10*0.025 ± 0.0030.010 ± 0.002
  • Data are means ± SE. Significant differences (P < 0.05) versus control (

  • *

    * ) and insuline (

  • ) groups. INS+AA, insulin in conjunction with amino acids.