TABLE 1

Effects of a long-term or short-term exposure of INS-1 cells to oleate on the ATP content and ATP-to-ADP ratio at low and high glucose

Culture conditions
ATP (nmole/μg DNA)ATP-to-ADP ratio
Glucose (mmol/l)2.8122.812
 Long-term
  BSA 72 h0.420 ± 0.0480.480 ± 0.043*4.9 ± 0.18.3 ± 0.9†
  Oleate 0.4 mmol/l 72 h0.500 ± 0.0480.510 ± 0.0566.4 ± 0.26.7 ± 0.5
 Short-term
  BSA 15 min0.296 ± 0.0330.355 ± 0.029‡NDND
  Oleate 0.4 mmol/l 15 min0.216 ± 0.0210.329 ± 0.029§NDND

  • Data are means ± SE of four independent experiments.

  • *

    * P ≤ 0.02 vs. 2.8 mmol/l glucose;

  • †

    † P ≤ 0.05 vs. 2.8 mmol/l glucose;

  • ‡

    ‡ P < 0.02 vs. 2.8 mmol/l glucose by paired analysis; and

  • §

    § P < 0.01 vs. 2.8 mmol/l glucose + oleate by paired analysis. ND, not determined.