TABLE 1

Phenotype and cytokine production of BBDR thymocytes after 5 days of culture

Cells added to ATOC
NoneFrozen isletsFrozen thyrocytes
Phenotype (%)
 CD4+CD8+11.4 ± 1.5%10.9 ± 1.2%9.5 ± 0.9%
 CD4+57.5 ± 3.1%60.1 ± 1.9%59.8 ± 1.9%
 CD8+27.5 ± 2.4%24.7 ± 2.0%26.5 ± 1.8%
 ART2a+47.8 ± 2.6%48.6 ± 1.7%48.0 ± 2.4%
 Transferrin receptor21.0 ± 3.4%23.0 ± 4.7%19.7 ± 2.7%
 IL-2R+ (CD25+)18.9 ± 3.0%20.4 ± 4.2%18.0 ± 1.6%
Elispot+ cell number/  2 × 105 cells
 IFN-γ49 ± 1232 ± 736 ± 7
 IL-450 ± 2265 ± 2237 ± 18
  • Data are means ± SD of six independent assays. Adult BBDR rat thymocytes were cultured in the presence or absence of islets and thyrocytes as indicated. Phenotyping data are expressed as the percentage of αβTCR+ cells present in the lymphocyte gate. Elispot data represent the number of cytokine-expressing cells per 2 × 105 cells in the cultures. There are no statistically significant differences among the three ATOC groups for any of the measured variables.