TABLE 2

Phenotype and cytokine production of rat lymphoid cells recovered from WAG-rnu/rnu adoptive recipients of cultured BBDR rat thymocytes

Cells added to ATOC
NoneFrozen isletsFrozen thyrocytes
Phenotype (% of αβTCR+ cells)
 CD4+
  Spleen cells73, 85 (2)81 ± 2 (5)82 ± 2 (3)
  Lymph node cells84 ± 6 (5)83 ± 4 (3)
 CD8+
  Spleen cells10, 7 (2)8 ± 2 (5)7 ± 1 (3)
  Lymph node cells23 ± 2 (5)27 ± 2 (3)
 IL-2R+ (CD25+)
  Spleen cells21, 31 (2)24 ± 3 (5)22 ± 2 (3)
  Lymph node cells23 ± 2 (5)27 ± 2 (3)
 Cytokine production (pg/ml)
 IFN-γ (lymph node cells)
  Nondiabetic1,288 ± 514 (3)795, 4,531 (2)
  Diabetic3,558 ± 871 (4)2,935 ± 700 (3)
 IFN-γ (spleen cells)
  Nondiabetic866, 1,056 (2)1,143 ± 502 (3)679 ± 481 (3)
  Diabetic1,649 ± 1,221 (4)1,714 ± 595 (3)
Elispot+ cell number/ 1 × 105 spleen cells
  IFN-γ
  Nondiabetic135, 175 (2)186 ± 45 (5)129 ± 75 (3)
  Diabetic189 ± 95 (4)277 ± 58 (3)
  IL-4
  Nondiabetic63, 160 (2)115 ± 47 (5)67 ± 47 (3)
  Diabetic87 ± 32 (4)97 ± 65 (3)
  • Data are means ± SD or raw data in cases where n = 2. Adult BBDR rat thymocytes were cultured for 5 days in the presence or absence of islets and thyrocytes as indicated, and recovered cells were then transfused into athymic WAG-rnu/rnu recipients. Lymph node and spleen cells were recovered from diabetic rats at the time of disease onset or from nondiabetic rats at the conclusion of the experiment. Phenotyping data are expressed as the percentage of αβTCR+ cells present in the lymphocyte gate. Elispot data represent the number of cytokine-expressing cells per 1 × 105 cells in the cultures. The number of recipients analyzed is given in parentheses. There are no statistically significant differences among the three ATOC groups for any of the measured variables.