Specific activity of hexokinase type I and type II isozymes in the fractions of human skeletal muscle homogenate
Fraction | Hexokinase I* (U/mU CrK) | Hexokinase II* (U/mU CrK) | Hexokinase I (% of total) | |
---|---|---|---|---|
Thermal inactivation | Chromatography | |||
Particulate | 0.52 ± 0.09 | 0.13 ± 0.03 | 81 ± 4 | 78 ± 8 |
Soluble | 0.03 ± 0.01 | 0.05 ± 0.02 | 37 ± 13 | 32 ± 13 |
Soluble + particulate | 0.55 ± 0.1 | 0.18 ± 0.03 | 76 ± 4 | 68 ± 7 |
* Data are means ± SD. On the base of thermal inactivation method