TABLE 2

Specific activity of hexokinase type I and type II isozymes in the fractions of human skeletal muscle homogenate

FractionHexokinase I* (U/mU CrK)Hexokinase II* (U/mU CrK)Hexokinase I (% of total)
Thermal inactivationChromatography
Particulate0.52 ± 0.090.13 ± 0.0381 ± 478 ± 8
Soluble0.03 ± 0.010.05 ± 0.0237 ± 1332 ± 13
Soluble + particulate0.55 ± 0.10.18 ± 0.0376 ± 468 ± 7
  • * Data are means ± SD. On the base of thermal inactivation method