TABLE 1

Insulin and proinsulin secretion and content of human islets of Langerhans cultured in NG5, HG5, and NG5+MAN5

NG5HG5NG5+MAN5
Chronic insulin release (nmol/l/mg/day)925.50 ± 129.10*2,843.00 ± 337.021,060.40 ± 186.50*
Basal insulin secretion (nmol/l/mg/min)0.0574 ± 0.0070.043 ± 0.0070.064 ± 0.004
Stimulated insulin release (nmol/l/mg/min)0.49 ± 0.0350.129 ± 0.0350.68 ± 0.07
Insulin content (nmol/l/mg/min)101 ± 2523.9 ± 5.7284.1 ± 16.7
Chronic proinsulin release (nmol/l/mg/day)0.26 ± 0.14§1.83 ± 0.1310.39 ± 0.12§
Proinsulin content (nmol/l/mg/min)15.10 ± 0.026.60 ± 0.0139.05 ± 0.03
  • Data are means ± SD of three separate experiments in three different human islet preparations and analyzed by ANOVA.

  • * NG5 vs. HG5 and NG5+MAN5 vs. HG5 (P < 0.01);

  • NG5 vs. HG5 and NG5+MAN5 vs. HG5 (P < 0.01);

  • NG5 vs. HG5, NG5+MAN5 vs. HG5, NG5 vs. NG5+MAN5 (P < 0.01);

  • § NG5 vs. HG5 and NG5+MAN5 vs. HG5 (P < 0.001);

  • NG5 vs. HG5, NG5+MAN5 vs. HG5, NG5 vs. NG5+MAN5 (P < 0.001).