TABLE 1

Insulin secretion by single β-cells in response to a 1-h incubation to NGF in different extracellular glucose concentrations

TreatmentGlucose (mmol/l)Percentage of plaque-forming cellsPlaque area (μmol/l)
Control5.641 ± 21,280 ± 113
15.651 ± 3*2,299 ± 119*
NGF5.656 ± 32,152 ± 160
15.666 ± 2*3,189 ± 198*
Nifedipine5.629 ± 41,078 ± 270
15.632 ± 21,160 ± 191
NGF + nifedipine5.632 ± 3§1,186 ± 174§
15.629 ± 4§1,828 ± 506§
  • Data are mean ± SE unless otherwise indicated.

  • *

    * Denotes significance level with respect to its own control in 5.6 mmol/l glucose, in both groups (P < 0.01). Significance level of experimental cells with respect to control cells in both glucose concentrations: P < 0.01

  • , P < 0.05

  • Significance level of NGF + nifedipine with respect to NGF-treated cells in both glucose concentrations: P < 0.01

  • §

    § ; n = 13 experiments by duplicate for control and NGF, and n = 5 for nifedipine alone or with NGF.