TABLE 1

Cytotoxic effect of oleate and palmitate in cultured rat β-cell preparations

Exposure time (mmol/l)Cytotoxicity
2 days8 days
Oleate0.125<57 ± 1*
0.2507 ± 2*13 ± 2
0.5009 ± 2*25 ± 2
Palmitate0.1255 ± 1*15 ± 2
0.25012 ± 243 ± 3
0.50054 ± 594 ± 2
Oleate0.125<5<5§
Palmitate0.125
Oleate0.2507 ± 113 ± 3
Palmitate0.250
Bromopalmitate0.1255 ± 1#37 ± 3
0.25013 ± 277 ± 1
0.50075 ± 6100 ± 0
  • Data are means ± SE for six to nine experiments. FFA-induced toxicity after 2 and 8 days of culture at 10 mmol/l glucose and 1% bovine serum albumin. Cytotoxicity of single fatty acids was assessed by comparison with control condition (no fatty acids added):

  • *

    * P < 0.01;

  • P < 0.001;

  • P < 0.01 vs. condition without palmitate;

  • §

    § P < 0.01;

  • P < 0.05;

  • P < 0.001 vs. condition without oleate;

  • #

    # P < 0.05. (analysis of variance, followed by paired t test with Bonferroni correction).