TABLE 2

Fatty acid induction of necrosis and apoptosis in β-cells

Exposure time (mmol/l)Cytotoxicity
2 days
8 days
NecrosisApoptosisNecrosisApoptosis
Oleate0.125<5<5<57 ± 2
0.250<5<510 ± 2*11 ± 2
0.500 9 ± 2*<514 ± 225 ± 4
Palmitate0.125<5<57 ± 2<5
0.250 9 ± 2*<59 ± 27 ± 2
0.50033 ± 5 7 ± 2*33 ± 526 ± 3
Bromopalmitate0.125<512 ± 2<552 ± 4
0.250<520 ± 3*<566 ± 3
0.50035 ± 5*27 ± 4*52 ± 624 ± 4*
  • Data are means ± SE for n experiments. Apoptosis and necrosis index for FFAs after 2 (n = 5–8) and 8 (n = 5–13) days of culture of β-cells in Ham’s F10 medium with 6 mmol/l glucose and 1% bovine serum albumin. Statistical significance (FFA conditions vs. control) was calculated by analysis of variance, followed by paired t test with the Bonferroni correction.

  • *

    * P < 0.01;

  • P < 0.001;

  • P < 0.05.