TABLE 3

FFA-induced apoptosis and necrosis in islet non–β-cells

Exposure time (mmol/l)Cytotoxicity
2 days
8 days
NecrosisApoptosisNecrosisApoptosis
Oleate0.125<55 ± 1*<524 ± 3*
0.250<58 ± 2<531 ± 5*
0.500<510 ± 3<527 ± 4*
Palmitate0.125<5<5<56 ± 4
0.250<5<5<517 ± 5
0.5005 ± 312 ± 2* 5 ± 235 ± 7*
Bromopalmitate0.125<510 ± 5<551 ± 5
0.250<513 ± 4<556 ± 6
0.5006 ± 322 ± 4*14 ± 539 ± 8*
  • Data are means ± SE for five experiments. Apoptosis and necrosis indexes after a 2- and 8-day FFA exposure of single non–β-cells in culture medium containing 6 mmol/l glucose, 1% bovine serum albumin, and 0.5 μg/ml growth hormone are shown. Statistical significance (FFA conditions vs. control) was calculated by analysis of variance A, followed by paired t test with the Bonferroni correction.

  • *

    * P < 0.01;

  • P < 0.05;

  • P < 0.001.