TABLE 2

Pulsatile insulin secretion

Pulse interval (min/pulse)Pulse amplitude (pmol/min)Pulse mass (pmol · kg−1 · pulse)Pulsatile secretion (pmol · kg−1 · min)Total insulin secretion (pmol · kg−1 · min)% Pulsatile insulin secretionInsulin clearance (l/min)
Meal (n = 7)
 Pre-alloxan
  Fasting6.5 ± 0.510.8 ± 3.4*24.0 ± 7.1*4.1 ± 1.55.9 ± 2.065.1 ± 5.10.85 ± 0.1*
  Fed5.8 ± 0.477.0 ± 28.2*203.8 ± 81.7*35.8 ± 13.5*42.4 ± 14.1*80.3 ± 3.7**1.08 ± 0.14**
 Post-alloxan
  Fasting5.8 ± 0.64.5 ± 1.412.6 ± 5.41.9 ± 0.63.2 ± 0.954.2 ± 4.00.67 ± 0.06
  Fed7.1 ± 0.819.7 ± 8.375.8 ± 36.012.7 ± 6.021.3 ± 8.454.1 ± 3.20.69 ± 0.14
Clamp (n = 5)
 Pre-alloxan
  Fasting7.3 ± 0.5*7.7 ± 0.7*22.4 ± 04.3**3.1 ± 0.54.8 ± 0.665.0 ± 6.90.89 ± 0.14
  Clamp5.5 ± 0.742.6 ± 17.7*80.9 ± 19.0**16.0 ± 5.5**31.1 ± 6.1**47.1 ± 7.80.64 ± 0.17
 Post-alloxan
  Fasting4.7 ± 0.34.2 ± 1.414.1 ± 6.43.1 ± 1.44.3 ± 1.465.2 ± 6.20.62 ± 0.04
  Clamp5.7 ± 0.53.4 ± 1.58.5 ± 4.31.5 ± 0.62.8 ± 0.645.0 ± 10.60.42 ± 0.08
  • Data are means ± SE. The calculated insulin secretion rate and parameters for pulsatile insulin secretion before and after alloxan administration after glucose injection and during the hyperglycemic clamp.

  • *

    * P < 0.05;

  • **

    ** P < 0.01 before versus after alloxan administration.