TABLE 1

Purification of FN3K

StepTotal protein (mg)Total activity* (units)Specific activityPurificationYield (%)
Crude lysate160,0005220.00331
DEAE-14,0006120.1545.5100
Ammonium sulfate ppt.1,0001300.1339.425
DEAE-228842146448
Gel filtration (Toyopearl-50)91826033.4
Affinity chromatography1.6148.82,6602.7
IEF0.032412538,0000.6
  • *

    * A unit of FN3K activity is defined as the micromoles per hour of FL phosphorylated to FL3P;

  • specific activity is defined as units of FN3k per milligram protein.