TABLE 2

NADH shuttle enzyme activities in fetal pig ICCs and adult islets

EnzymenFetal pigAdult pigP
mGPDH, ICCs (day 3)4–87.2 ± 1.233.1 ± 1.1<0.001
mGPDH, ICCs (day 0)61.7 ± 0.2
mGPDH, β-cells154.7 ± 0.8
cGPDH (day 3)3–8186 ± 62106 ± 110.07
cGPDH, ICCs (day 0)65.3 ± 0.7
cGPDH, β-cells847.6 ± 8.1
mMDH, ICCs (day 3)6462 ± 722525 ± 124<0.001
mMDH, β-cells8284 ± 38.5
cMDH, ICCs (day 3)52126 ± 3451172 ± 1150.03
cMDH, β-cells8650 ± 73
  • Data are means ± SE. Enzyme activities were measured after allowing the islets to recover overnight from the isolation procedure. Units for mGPDH enzyme activity are nanomoles of substrate (2-p-iodo-3-nitro-5-phenyltetrazolium) reduced per minute per milligram protein; for cGPDH and cMDH nanomoles of NADH reduced per minute per milligram protein; and for mMDH nanomoles of NADH oxidized per minute per milligram protein. n, number of experiments/islet preparations. Duplicate samples were taken for each experiment/islet preparation.