TABLE 3

Glucose-stimulated insulin secretion of infected islets in vitro

Treatment
Virus3 mmol/l glucose20 mmol/l glucose3 mmol/l glucose
Neg295 ± 26.21241 ± 26.2327 ± 23.3
Ad-LacZ237 ± 21.91201 ± 27.6260 ± 39.6
Ad-IDO288 ± 14.81174 ± 42.4271 ± 26.2
  • Data are means ± SD. In this experiment, shown as an example, groups of 25 islets were infected with Ad-LacZ and Ad-IDO. Infected islets were compared with uninfected (Neg) islets by culturing them for 4 days before being pulsed with media containing high and low glucose levels. Media were collected after a 30-min exposure to islets. Insulin content measured by ELISA is expressed as picogram insulin per milliliter of media.