TABLE 4

Tryptophan levels in a “head start” proliferation assay

100 μl media
200 μl media
NegAd-LacZAd-IDONegAd-LacZAd-IDO
Day 514.8 ± 0.314.2 ± 1.98.7 ± 0.419.0 ± 3.119.2 ± 0.621.6 ± 0.6
Day 614.3 ± 1.115.7 ± 2.110.3 ± 5.619.9 ± 2.120.7 ± 3.119.0 ± 2.7
Day 716.2 ± 1.316.9 ± 1.18.3 ± 2.024.7 ± 2.222.3 ± 2.116.9 ± 5.1
Day 816.4 ± 0.214.4 ± 0.37.0 ± 0.816.7 ± 0.619.0 ± 0.115.8 ± 1.4
  • Data are means ± SD. Islets not cultured with spleen cells were plated in parallel to islets co-cultured with spleen cells to test tryptophan levels of culture media (nmole/ml) on days corresponding to T-cell proliferation measurements in media volumes of 100 and 200 μl per well. Uninfected islets (Neg) were compared with islets infected with Ad-LacZ or Ad-IDO.