TABLE 1

Sequences of oligonucleotide primers and PCR conditions

Gene Name	Size (bp)	5′ Oligonucleotide	3′ Oligonucleotide	GenBank accession no.	MgCl₂ (mmol/l)	dNTP (μmol/l)	Primer (nmol/l)	Annealing (°C)	Cycles	Control gene
HO-I	307	ATG CCC CGC TCT ACT TCC	GTG GGG TTG TCG ATC CTC	J02722	1.5	160	500	62	27	Cyclophilin
Glutathione peroxidase	301	GGT TTC CCG TGC AAT CAG	GAA CAC CGT CTG GAC CTA CC	X07365	1.5	80	200	59	27	TBP
Cu/Zn-SOD	333	AAC TGA AGG CGA GCA TGG	ATC ACA CCA CAA GCC AAGC	M21060	1.5	80	200	56	23	α-Tubulin
Mn-SOD	410	CAC CAC AGC AAG CAC CAC	TCC CAC ACA TCA ATC CCC	Y00497	1.5	160	80	62	26	TBP
Catalase	173	GCT CCC AAC TAC TAC CCC AAC	CGT TTC CTC TCC TCC TCA TTC	M11670	1.5	160	100	62	27	α-Tubulin
A20	497	TTT GAG CAA TAT GCG GAA AGC	AGT TGT CCC ATT CGT CAT TCC	U19463	2.0	80	400	55	30	TBP
BCL-2	170	CCT GGC ATC TTC TCC TTC	AGA AGT CAT CCC CAG CCC	L14680	1.5	120	400	62	27	Cyclophilin
HSP70	278	ACG CAG ACC TTC ACC ACC	CGC TCG ATC TCC TCC TTG	X75357	1.5	160	200	62	28	TBP
NF-κB (subunit p65)	144	TTT CCC CTC ATC TTT CCC TC	TGT GCT TCT CTC CCC AGG	AF079314	1.5	160	400	62	26	TBP
p53	295	GCT CAC TCC AGC TAC CCG	ACC CAG CAA CTA CCA ACC C	X13058	1.5	160	150	62	27	α-Tubulin
Fas	322	TGA GCC TTG GAG ACG AAC	ATC TTG GGG GCT GTT GTG	D26112	1.5	160	400	62	30	TBP
Cyclophilin	400	AAC CCC ACC GTG TTC TTC	TGC CTT CTT TCA CCT TCC C	M19533	1.5	120–160	40	62
TBP	190	ACC CCT CAC CAA TGA CTC CTA TG	ATG ATG ACT GCA GCA AAT CGC	D01034	1.5–2.0	80–160	100–200	55–62
α-Tubulin	451	CTC GCA TCC ACT TCC CTC	ATG CCC TCA CCC ACG TAC	J00797	1.5	160	200	56–62