Sequences of oligonucleotide primers and PCR conditions
Gene Name | Size (bp) | 5′ Oligonucleotide | 3′ Oligonucleotide | GenBank accession no. | MgCl2 (mmol/l) | dNTP (μmol/l) | Primer (nmol/l) | Annealing (°C) | Cycles | Control gene |
---|---|---|---|---|---|---|---|---|---|---|
HO-I | 307 | ATG CCC CGC TCT ACT TCC | GTG GGG TTG TCG ATC CTC | J02722 | 1.5 | 160 | 500 | 62 | 27 | Cyclophilin |
Glutathione peroxidase | 301 | GGT TTC CCG TGC AAT CAG | GAA CAC CGT CTG GAC CTA CC | X07365 | 1.5 | 80 | 200 | 59 | 27 | TBP |
Cu/Zn-SOD | 333 | AAC TGA AGG CGA GCA TGG | ATC ACA CCA CAA GCC AAGC | M21060 | 1.5 | 80 | 200 | 56 | 23 | α-Tubulin |
Mn-SOD | 410 | CAC CAC AGC AAG CAC CAC | TCC CAC ACA TCA ATC CCC | Y00497 | 1.5 | 160 | 80 | 62 | 26 | TBP |
Catalase | 173 | GCT CCC AAC TAC TAC CCC AAC | CGT TTC CTC TCC TCC TCA TTC | M11670 | 1.5 | 160 | 100 | 62 | 27 | α-Tubulin |
A20 | 497 | TTT GAG CAA TAT GCG GAA AGC | AGT TGT CCC ATT CGT CAT TCC | U19463 | 2.0 | 80 | 400 | 55 | 30 | TBP |
BCL-2 | 170 | CCT GGC ATC TTC TCC TTC | AGA AGT CAT CCC CAG CCC | L14680 | 1.5 | 120 | 400 | 62 | 27 | Cyclophilin |
HSP70 | 278 | ACG CAG ACC TTC ACC ACC | CGC TCG ATC TCC TCC TTG | X75357 | 1.5 | 160 | 200 | 62 | 28 | TBP |
NF-κB (subunit p65) | 144 | TTT CCC CTC ATC TTT CCC TC | TGT GCT TCT CTC CCC AGG | AF079314 | 1.5 | 160 | 400 | 62 | 26 | TBP |
p53 | 295 | GCT CAC TCC AGC TAC CCG | ACC CAG CAA CTA CCA ACC C | X13058 | 1.5 | 160 | 150 | 62 | 27 | α-Tubulin |
Fas | 322 | TGA GCC TTG GAG ACG AAC | ATC TTG GGG GCT GTT GTG | D26112 | 1.5 | 160 | 400 | 62 | 30 | TBP |
Cyclophilin | 400 | AAC CCC ACC GTG TTC TTC | TGC CTT CTT TCA CCT TCC C | M19533 | 1.5 | 120–160 | 40 | 62 | ||
TBP | 190 | ACC CCT CAC CAA TGA CTC CTA TG | ATG ATG ACT GCA GCA AAT CGC | D01034 | 1.5–2.0 | 80–160 | 100–200 | 55–62 | ||
α-Tubulin | 451 | CTC GCA TCC ACT TCC CTC | ATG CCC TCA CCC ACG TAC | J00797 | 1.5 | 160 | 200 | 56–62 |