TABLE 1

Sequences of oligonucleotide primers and PCR conditions

Gene NameSize (bp)5′ Oligonucleotide3′ OligonucleotideGenBank accession no.MgCl2 (mmol/l)dNTP (μmol/l)Primer (nmol/l)Annealing (°C)CyclesControl gene
HO-I307ATG CCC CGC TCT ACT TCCGTG GGG TTG TCG ATC CTCJ027221.51605006227Cyclophilin
Glutathione peroxidase301GGT TTC CCG TGC AAT CAGGAA CAC CGT CTG GAC CTA CCX073651.5802005927TBP
Cu/Zn-SOD333AAC TGA AGG CGA GCA TGGATC ACA CCA CAA GCC AAGCM210601.5802005623α-Tubulin
Mn-SOD410CAC CAC AGC AAG CAC CACTCC CAC ACA TCA ATC CCCY004971.5160806226TBP
Catalase173GCT CCC AAC TAC TAC CCC AACCGT TTC CTC TCC TCC TCA TTCM116701.51601006227α-Tubulin
A20497TTT GAG CAA TAT GCG GAA AGCAGT TGT CCC ATT CGT CAT TCCU194632.0804005530TBP
BCL-2170CCT GGC ATC TTC TCC TTCAGA AGT CAT CCC CAG CCCL146801.51204006227Cyclophilin
HSP70278ACG CAG ACC TTC ACC ACCCGC TCG ATC TCC TCC TTGX753571.51602006228TBP
NF-κB (subunit p65)144TTT CCC CTC ATC TTT CCC TCTGT GCT TCT CTC CCC AGGAF0793141.51604006226TBP
p53295GCT CAC TCC AGC TAC CCGACC CAG CAA CTA CCA ACC CX130581.51601506227α-Tubulin
Fas322TGA GCC TTG GAG ACG AACATC TTG GGG GCT GTT GTGD261121.51604006230TBP
Cyclophilin400AAC CCC ACC GTG TTC TTCTGC CTT CTT TCA CCT TCC CM195331.5120–1604062
TBP190ACC CCT CAC CAA TGA CTC CTA TGATG ATG ACT GCA GCA AAT CGCD010341.5–2.080–160100–20055–62
α-Tubulin451CTC GCA TCC ACT TCC CTCATG CCC TCA CCC ACG TACJ007971.516020056–62