TABLE 1

Kinetic characteristics of GK A456V

Kinetic parameterWild-type GKA456VGK
kcat (s−1)53.2 ± 0.9784.9 ± 4.5
Glucose S0.5 (mmol/l)8.04 ± 0.282.53 ± 0.10
nH (unitless)1.67 ± 0.041.21 ± 0.03
ATP Km (mmol/l)
 At glucose S0.50.24 ± 0.020.15 ± 0.01
 At 10 times the glucose S0.50.39 ± 0.020.29 ± 0.03
Relative activity index (unitless)1.037.9 ± 1.23
  • Data are means ± SE. The results are the means of the kinetic analysis of four independent expressions of wild-type and mutant GST-GK. Differences between the mutant and wild-type enzyme are statistically significant for all parameters studied (P ≤ 0.05).