TABLE 1

Summary of studies that present results consistent with the view that insulin-secreting cells possess RY receptors

Insulin-secreting cellsMain methods/pharmacological toolsReference
RINm5F cellsThimerosal, permeabilized cells(5)
Fura-2, caffeine(24)
RT-PCR(30)
INS-1 cellsFura-2, caffeine(25,26)
ER aequorin, 4-chloro-m-cresol(120)
Western blot(25)
βTC3 cellsRT-PCR(19)
RNAse protection assay(16)
Fura-2, ryanodine(66)
MIN6 cellsVesicle-targetted aequorin, caffeine, cADPR, 4-chloro-3-ethylphenol(22)
Immunocytochemistry(121)
Fluorescent ryanodine(121)
ER-targeted cameleon, caffeine(82,121)
Fluorescent ryanodine(82)
Caged cADPR(82)
HIT-T15 cellsFura-2, caffeine(19,64)
Fura-2, 4-chloro-m-cresol(64)
RT-PCR(27)
Fluo-3, Ruthenium red, X-ray microanalysis(114)
Mouse isletsFura-2, caffeine(59)
RT-PCR(18)
RNAse protection assay(16)
Microsomes, 45Ca2+, theophylline(46)
Mouse β-cellsFura-2, caffeine(16,55)
Fura-2, caffeine, ryanodine(67)
Fura-2, 4-chloro-3-ethylphenol(16)
Fluo-3, caffeine(58)
Fluorescent ryanodine(19)
Rat Islets45Ca2+ efflux, theophylline(10)
Microsomes, fluo-3, cADPR, ryanodine(15)
RT-PCR(19)
Rat β-cellsFura-2, caffeine(55,72,122)
Fluo-3, caffeine, ryanodine, NO(17)
Fluorescent ryanodine(19)
Human β-cellsFluorescent ryanodine imaging(19)
Quantitative ryanodine-binding(19)