Amount of dead islet cells after 48-h incubation with control culture medium or medium containing 2.0 mmol/l FFA mixture
Amount of dead cells | ||
---|---|---|
Controls | FFA exposed | |
TUNEL (%) | 8.9 ± 2.0 | 24.0 ± 4.0* |
ELISA (OD) | 0.9 ± 0.1 | 2.9 ± 0.6* |
Data are means ± SD. TUNEL experiments were from four separate pancreata; ELISA experiments were from nine separate pancreata.
* P < 0.01 vs. controls.