TABLE 1

Densitometric analysis after in situ hybridization of glucocorticoid receptor mRNA expression in the limbic system (hippocampal CA1/2, CA3, and CA4 fields and dentate gyrus), the hypothalamus (arcuate nucleus, PVN, and ventromedial hypothalamus) and the pars distalis of normal, STZ-induced diabetic, and insulin-treated diabetic rats under basal conditions and after insulin-induced hypoglycemia

Normal
STZ
STZ + insulin
BasalHypoglycemiaBasalHypoglycemiaBasalHypoglycemia
n6665610
Limbic system
 CA1/215.0 ± 2.510.0 ± 1.313.5 ± 2.811.3 ± 0.520.0 ± 1.812.0 ± 0.5
 CA37.8 ± 0.86.5 ± 0.56.8 ± 0.58.3 ± 0.58.8 ± 0.3*6.6 ± 0.3
 CA48.0 ± 1.56.5 ± 0.36.8 ± 0.87.8 ± 0.88.8 ± 0.56.8 ± 0.3
 DG15.5 ± 2.311.3 ± 1.313.8 ± 2.314.8 ± 0.519.8 ± 1.811.3 ± 0.8
Hypothalamus
 ARC9.0 ± 1.59.5 ± 7.810.0 ± 0.815.8 ± 3.810.3 ± 1.514.0 ± 1.3
 PVN11.5 ± 0.759.8 ± 0.510.5 ± 1.312.0 ± 1.017.8 ± 2.513.8 ± 0.8
 VMH8.0 ± 0.514.0 ± 2.06.5 ± 0.515.0 ± 1.58.3 ± 0.815.3 ± 1.0
Pars distalis7.3 ± 1.510.3 ± 1.06.8 ± 0.511.0 ± 1.025.5 ± 1.525.0 ± 1.0
  • Data are means ± se of ROD. ARC, arcuate nucleus, DG, dentate gyrus, VMH, ventromedial hypothalamus.

  • *

    * P < 0.05 vs. STZ − basal;

  • P < 0.05 vs. normal − basal and STZ − basal.