TABLE 3

Comparison of leptin secretion and mRNA expression between omental and subcutaneous preadipocytes differentiated for 16 days in the presence or absence of rosiglitazone

n−Rosiglitazone
+Rosiglitazone
OMSCP (OM vs. SC)OMSCP (OM vs. SC)
Leptin secretion
 A) ng/(1 × 24 h)2214 ± 227 ± 7<0.0523 ± 4*58 ± 13*<0.001
 B) μg/(g total RNA × 24 h)98 ± 267 ± 25<0.0116 ± 4104 ± 35<0.01
 C) pg/(mU GPDH × μg protein × 24 h)7NDND23 ± 639 ± 9<0.05
Leptin mRNA expression
 D) pmol mRNA/g total RNA918 ± 969 ± 30<0.0515 ± 623 ± 7*NS
  • Data are means ± SE. Leptin secretion is expressed as A) absolute concentration released into the medium, B) A corrected for differences in total cell amount expressed as amount of total RNA, C) A corrected for differentiation expressed as GPDH activity, and D) leptin mRNA expression was expressed as pmol mRNA per gram of total RNA. Leptin secretion and mRNA expression were statistically compared between omental (OM) and subcutaneous (SC) preadipocytes. NS, nonsignificant. Leptin secretion and mRNA expression were compared between cells differentiated with (+ rosiglitazone) or without rosiglitazone (− rosiglitazone):

  • *

    * P < 0.05. n, number of experiments. ND, GPDH activity was below the detection limit.