TABLE 4

Comparison of TNF-α secretion and mRNA expression between omental and subcutaneous preadipocytes differentiated for 16 days in the presence or absence of rosiglitazone

n−Rosiglitazone
+Rosiglitazone
OMSCP (OM vs. SC)OMSCP OM vs. SC
TNF-α secretion
 A) ng/(1 × 24 h)220.25 ± 0.070.16 ± 0.04<0.050.16 ± 0.03*0.13 ± 0.04NS
 B) ng/(g total RNA × 24 h)933 ± 835 ± 14NS4 ± 2*8 ± 4*NS
 C) pg/(mU GPDH × μg protein      × 24 h)7NDND0.31 ± 0.080.26 ± 0.05NS
TNF-α mRNa expression
 D) pmol mRNA/g total RNA90.61 ± 0.0060.56 ± 0.011<0.050.54 ± 0.005*0.54 ± 0.008NS