TABLE 1

IC50 of oxidative phosphorylation inhibitors

Proinsulin biosynthesisTotal protein synthesisInsulin secretionATP content
Rotenone (nmol/l)39.0 ± 2.5 (3)39.0 ± 1.0 (3)13.2 ± 4.3 (6)43.3 ± 6.2 (3)
Antimycin A1 (nmol/l)31.3 ± 9.0 (3)43.3 ± 7.5 (3)218 ± 54 (5)54.7 ± 7.4 (3)
DNP (μmol/l)29.3 ± 2.7 (3)32.3 ± 5.5 (3)2.0 ± 0.3 (5)33.3 ± 11.7 (3)
Oligomycin (ng/ml)40 ± 10 (3)60 ± 10 (4)180 ± 40 (7)170 ± 70 (3)
  • Values are means ± SE (number of replicates). Islets were preincubated at basal (2.8 mmol/l) glucose for 1 h and then incubated at 16.7 mmol/l glucose in the presence of increasing concentrations of the indicated inhibitor for an additional hour. Proinsulin biosynthesis, total protein synthesis, insulin secretion, and islet ATP content were determined as stated in research design and methods.