Succinate and malate content in glucose-stimulated rat pancreatic islets
| Succinate (pmol/μg of DNA) | Malate (pmol/μg of DNA) | |
|---|---|---|
| n | 5 | 4 |
| 2.8 mmol/l glucose | 100.6 ± 4.8 | 52.1 ± 6.2 |
| 16.7 mmol/l glucose | 140.1 ± 11.5* | 61.3 ± 8.1 |
| 16.7 mmol/l glucose + 1 mmol/l malonate methyl ester | 147.0 ± 16.9* | 58.7 ± 10.0 |
Values are means ± SE. Isolated islets were preincubated for 1 h at 2.8 mmol/l glucose, followed by 1-h incubation in the presence of the indicated agent. Succinate and malate were extracted from islets with TCA and analyzed using a fluorometric enzyme assay as described previously (24) and in research design and methods.
* P < 0.05 vs. 2.8 mmol/l glucose.