TABLE 1

Effects of pancreatic islet chronic exposure (48 h) to high glucose on ATP levels and the ATP/ADP ratio

Culture conditionsGlucose 2.8 mmol/lGlucose 16.7 mmol/lΔ Over basal
Glucose 5.5 mmol/l
 ATP (pmol/islet)25.7 ± 1.737.7 ± 1.812.0 ± 0.6
 ADP (pmol/islet)5.5 ± 0.53.8 ± 0.7
 ATP/ADP ratio4.8 ± 0.510.1 ± 1.4
Glucose 16.7 mmol/l
 ATP (pmol/islet)35.5 ± 2.5*36.2 ± 2.30.7 ± 0.5
 ADP (pmol/islet)7.9 ± 1.18.0 ± 1.0*
 ATP/ADP ratio4.5 ± 1.14.5 ± 0.9*
  • Data are mean ± SE of three separate experiments.

  • *

    * P < 0.05,

  • P < 0.01 vs. controls;

  • P < 0.05 vs. 2.8 mmol/l glucose.