TABLE 2

Effects of pancreatic islet chronic exposure (72 h) to high FFA on ATP levels and the ATP/ADP ratio

Culture conditions 72 hGlucose 2.8 mmol/lGlucose 16.7 mmol/lΔ Over basal
BSA 2%, glucose 5.5 mmol/l
 ATP (pmol/islet)30.7 ± 1.742.2 ± 2.012.1 ± 1.5
 ADP (pmol/islet)4.6 ± 0.33.1 ± 0.2
 ATP/ADP ratio6.7 ± 0.513.8 ± 1.5
FFA 2 mmol/l, glucose 5.5 mmol/l
 ATP (pmol/islet)38.5 ± 1.9*38.1 ± 0.80.5 ± 0.3
 ADP (pmol/islet)6.5 ± 0.96.6 ± 1.0*
 ATP/ADP ratio6.0 ± 0.95.8 ± 0.8*
  • Data are mean ± SE of three separate experiments.

  • *

    * P < 0.05,

  • P < 0.01 vs. controls;

  • P < 0.05 vs. 2.8 mmol/l glucose.