TABLE 3

Effects of pancreatic islet chronic exposure (72 h) to high FFA with or without BADGE on ATP levels and the ATP/ADP ratio

Culture conditions 72 hGlucose 2.8 mmol/lGlucose 16.7 mmol/lΔ Over basal
BSA 2%, glucose 5.5 mmol/l
 ATP (pmol/islet)32.4 ± 2.444.4 ± 3.111.9 ± 1.8
 ADP (pmol/islet)5.1 ± 0.53.4 ± 0.4
 ATP/ADP ratio6.3 ± 0.613.1 ± 1.1§
FFA 2 mmol/l, glucose 5.5 mmol/l
 ATP (pmol/islet)38.8 ± 2.539.9 ± 1.71.15 ± 0.8
 ADP (pmol/islet)5.9 ± 0.45.5 ± 0.6
 ATP/ADP ratio6.6 ± 0.87.3 ± 0.8*
FFA 2 mmol/l, glucose 5.5 mmol/l + BADGE 100 μmol/l
 ATP (pmol/islet)42.5 ± 1.651.8 ± 1.89.3 ± 1.3
 ADP (pmol/islet)6.4 ± 0.54.6 ± 0.5
 ATP/ADP ratio6.6 ± 0.711.2 ± 1.1
BSA 2% + glucose 5.5 mmol/l BADGE 100 μmol/l
 ATP (pmol/islet)43.2 ± 1.956.8 ± 2.0§13.5 ± 1.9
 ADP (pmol/islet)6.4 ± 0.64.4 ± 0.4
 ATP/ADP ratio6.8 ± 0.712.9 ± 0.9§
  • Data are mean ± SE of four separate experiments.

  • *

    * P < 0.05,

  • P < 0.01

  • P < 0.05,

  • §

    § P < 0.01 vs. 2.8 mmol/l glucose.