TABLE 1

List of sequences of primers and probes used

StandardTypeSequenceGenBank number
 β-ActinForwardAGC CAT GTA CGT AGC CAT CCANM_007393
ReverseTCT CCG GAG TCC ATC ACA ATG
ProbeTGT CCC TGT ATG CCT CTG GTC GTA CCAC
Transcription factors
Srebp-1cForwardGGA GCC ATG GAT TGC ACA TTBI656094
ReverseCCT GTC TCA CCC CCA GCA TA
ProbeCAG CTC ATC AAC AAC CAA GAC AGT GAC TTC C
Srebp-2ForwardCTG CAG CCT CAA GTG CAA AGAF374267
ReverseCAG TGT GCC ATT GGC TGT CT
ProbeCCA TCC AGC AGC AGG TGC AGA CG
PparForwardTAT TCG GCT GAA GCT GGT GTA CX57638
ReverseCTG GCA TTT GTT CCG GTT CT
ProbeCTG AAT CTT GCA GCT CCG ATC ACA CTT G
PparForwardCAC AAT GCC ATC AGG TTT GGX57638
ReverseGCT GGT CGA TAT CAC TGG AGA TC
probeCCA ACA GCT TCT CCT TCT CGG CCT G
LxrForwardGCT CTG CTC ATT GCC ATC AGAF085745
ReverseTGT TGC AGC CTC TCT ACT TGG A
ProbeTCT GCA GAC CGG CCC AAC GTG
ChrebpForwardGAT GGT GCG AAC AGC TCT TCTAF156604
ReverseCTG GGC TGT GTC ATG GTG AA
ProbeCCA GGC TCC TCC TCG GAG CCC
DNL, cholesterogenisis, and β-oxidation
FasForwardGGC ATC ATT GGG CAC TCC TTAF127033
ReverseGCT GCA AGC ACA GCC TCT CT
ProbeCCA TCT GCA TAG CCA CAG GCA ACC TC
AccForwardGCC ATT GGT ATT GGG GCT TACAF374170
ReverseCCC GAC CAA GGA CTT TGT TG
ProbeCTC AAC CTG GAT GGT TCT TTG TCC CAG C
HmgrForwardCCG GCA ACA ACA AGA TCT GTGBB664708
ReverseATG TAC AGG ATG GCG ATG CA
ProbeTGT CGC TGC TCA GCA CGT CCT CTT C
CptIaForwardCTC AGT GGG AGC GAC TCT TCAAF017175
ReverseGGC CTC TGT GGT ACA CGA CAA
ProbeCCT GGG GAG GAG ACA GAC ACC ATC CAA C
McadForwardGCA GCC AAT GAT GTG TGC TTA CNM_007382
ReverseCAC CCT TCT TCT CTG CTT TGG T
ProbeCCC TCC GCA GGC TCT GAT GTG G
HmgsForwardTGG TGG ATG GGA AGC TGT CTAU12790
ReverseTTC TTG CGG TAG GCT GCA TAG
ProbeCCA AGG CCC GCA GGT AGC ACT G
VLDL metabolism
ApobForwardGCC CAT TGT GGA CAA GTT GAT CAW012827
ReverseCCA GGA CTT GGA GGT CTT GGA
ProbeAAG CCA GGG CCT ATC TCC GCA TCC
Apobec-IForwardTCG TCC GAA CAC CAG ATG CTNM_031159
ReverseGGT GTC GGC TCA GAA ACT CTG T
ProbeCCT GGT TCC TGT CCT GGA GTC CCT G
ApoeForwardCCT GAA CCG CTT CTG GGA TTNM_009696
ReverseGCT CTT CCT GGA CCT GGT CA
ProbeAAA GCG TCT GCA CCC AGC GCA GG
Dgat-IForwardGGT GCC GTG ACA GAG CAG ATNM_010046
ReverseCAG TAA GGC CAC AGC TGC TG
ProbeCTG CTG CTA CAT GTG GTT AAC CTG GCC A
MttpForwardCAA GCT CAC GTA CTC CAC TGA AGNM_008642
ReverseTCA TCA TCA CCA TCA GGA TTC CT
ProbeACG GCA AGA CAG CGT GGG CTA CA
Insulin signaling
IrForwardTGA GTC AGC CAG TCT TCG AGA ANM_010568
ReverseACT ACC AGC ATT GGC TGT CCT T
ProbeCTG CCA TCA TGT GGT CCH CCT TCT
Irs-1ForwardAGC ACC TGG TGG CTC TCT ACANM_010570
ReverseCAG CTG CAG AAG AGC CTG GTA
ProbeCTC GCT ATC CGC GGC AAT GGC
Irs-2ForwardAGT CCC ACA TCG GGC TTG AAGAF090738
ReverseGGT CTG CAC GGA TGA CCT TAG
ProbeCCT TCA AGT CAG CCA GCC CCC TG