TABLE 3

Acetyl-CoA pool enrichment and fractional palmitate synthesis values of adipose tissue and liver and hepatic acetyl-CoA pool enrichment and fractional cholesterol synthesis values in [1-13C]-acetate–enriched diet–fed lean and ob/ob mice

Leanob/ob
DNL
 Liver
  Acetyl-CoA pool enrichment (%)6.3 ± 0.35.9 ± 0.8
  Fractional hepatic palmitate (%)31.0 ± 6.153.1 ± 4.7*
  Hepatic palmitate (μmol/liver)1.1 ± 0.46.3 ± 0.9*
  Newly synthesized hepatic palmitate (μmol/liver)0.3 ± 0.13.4 ± 0.6*
 Adipose tissue
  Acetyl-CoA pool enrichment (%)1.6 ± 0.5ND
  Fractional synthesis adipose tissue palmitate (%)21.5 ± 8.7ND
Cholesterol synthesis
 Acetyl-CoA pool enrichment (%)6.3 ± 0.15.5 ± 0.5
 Fractional synthesis hepatic free cholesterol (%)17.8 ± 4.012.6 ± 3.4*
 Hepatic free cholesterol (μmol/liver)1.7 ± 0.12.7 ± 0.5*
 Newly synthesized hepatic cholesterol (μmol/liver)0.3 ± 0.10.3 ± 0.2
  • Data are means ± SD. n = 5 lean and 5 ob/ob mice.

  • *

    * P < 0.05, Mann-Whitney U test. ND, not detectable.