TABLE 4

Oligonucleotides and probes used in 5′ nuclease assays of IL-1β, IL-4, IL-10, TNF-α, and β-actin

GeneAccession number GenBankForward primer sequence5′ nuclease probe (labeled with 5′-FAM and 3′-TAMRA)Reverse primer sequencePCR product (bp)
IL-1βNM_0083615′TGT CCT GTG TGA TGA AAG ACG G3′5′ACA CCC ACC CTG CAG CTG GAG AGT3′5′TTG GGT ATT GTT TGG GAT CCA3′69
IL-4M258925′ACA GGA GAA GGG ACG CCA T3′5′TCC TCA CAG CAA CGA AGA ACA CCA CA3′5′GAA GCC CTA CAG ACG AGC TCA3′95
IL-10M378975′TGT GAA AAT AAG AGC AAG GCA GTG3′5′AGC AGG TGA AGA GTG ATT TTA ATA AGC TCC AAG ACC3′5′CAT TCA TGG CCT TGT AGA CAC C3′85
TNF-αM117315′GAC CCT CAC ACT CAG ATC ATC TTC T3′5′TAG CCC ACG TCG TAG CAA ACC ACC AA 3′5′CGC TGG CTC AGC CAC TCC3′105
β-actinX036725′GCT CTG GCT CCT AGC ACC3′5′TGA AGA TCA AGT CAT TGC TCC TCC TGA GC3′5′CCA CCT ATC CAC ACA GAG TAC TTG3′73