The effect of DC-41-33 on hormonal release in perfused pancreas
| Min | DC-41-33 (μmol/l) | 3.3 mmol/l glucose | 5.5 mmol/l glucose | 20% of the standard Ca2+ 3.3 mmol/l glucose | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Insulin | Glucagon | Insulin | Glucagon | Insulin | Glucagon | |||||
| 0–4 | 0 | 14.4 ± 2.9 | 9.6 ± 2.2 | 30.6 ± 5.0 | 4.7 ± 1.2 | 8.1 ± 1.5 | 8.2 ± 1.6 | |||
| 0–4 | 1 | 9.8 ± 1.3 | 16.9 ± 2.2 | 24.7 ± 8.3 | 7.0 ± 1.2 | 3.7 ± 1.4 | 6.3 ± 1.4 | |||
| 4–20 | 0 | 38.2 ± 6.2 | 33.3 ± 7.5 | 89.0 ± 14.6 | 19.1 ± 5.5 | 12.7 ± 2.3 | 28.1 ± 8.2 | |||
| 4–20 | 1 | 38.2 ± 7.2 | 91.1 ± 20.2* | 119.8 ± 36.3 | 47.0 ± 6.1† | 14.1 ± 3.6 | 32.8 ± 7.4 | |||
| 0–20 | 0 | 52.6 ± 8.7 | 43.0 ± 9.6 | 119.7 ± 18.8 | 23.4 ± 6.4 | 20.8 ± 3.3 | 36.3 ± 9.5 | |||
| 0–20 | 1 | 47.9 ± 8.4 | 108.0 ± 22.2* | 144.5 ± 44.4 | 54.1 ± 7.2‡ | 17.7 ± 4.7 | 39.1 ± 8.8 | |||
Data were obtained from the perfusion experiments shown in Figs. 4–6 and represent the amounts of insulin (pmol) and glucagon (ng) released during the 20-min stimulation period. Arginine (20 mmol/l) was used as the stimulus. The hormone amount is calculated as the area under the curve, using the secretion rate at min 0 as the basal value.
* P < 0.02,
† P < 0.005, and
‡ P < 0.01 vs. without DC-41-33.