TABLE 1

Primers used and PCR conditions

GenesSize (bp)Primer Sequence (5′-3′)Annealing temperature (°C)MgCl2 (mmol/l)
p22PHOX435Forward-GCTCATCTGTCTGCTGGAGTA591.5
Reverse-ACGACCTCATCTGTAACTGGA
p47PHOX374Forward-AGGTGGTATGATGGGCAGCGTG591.5
Reverse-CCTCGGTTTGGCTTCATCTGGC
gp91PHOX336Forward-TGACTCGGTTGGCTGGCATC551.5
Reverse-CGCAAAGGTACAGGAACATGGG
β-actin549Forward-GTGGGGCGCCCCAGGCAGCA551.5
Reverse-CTCCTTAATGTCACGCACGATTTC