TABLE 2

Basal glucose and glycerol turnover rates in conscious control and knockout mice

ControlKnockout
Plasma glucose (mmol/l)5.9 ± 0.46.9 ± 0.2*
[2-3H]glucose turnover (μmol · kg−1 · min−1)61.7 ± 3.951.1 ± 2.3
[6-3H]glucose turnover (μmol · kg−1 · min−1)51.4 ± 2.144.2 ± 1.6*
[U-14C]glucose turnover (μmol · kg−1 · min−1)46.4 ± 3.339.8 ± 2.2
[d5]glycerol turnover (μmol · kg−1 · min−1)34.9 ± 2.331.4 ± 2.8
  • Data are means ± SE. In experiment 1, [2-3H]glucose, [6-3H]glucose and [U-14C]glucose were infused for 220 min. In experiment 2, [d5]glycerol with or without [6-3H]glucose was infused for 220 min. Male mice ages 7–8 months were fasted for 26 h before infusions.

  • *

    * P < 0.05 versus control values. n = 4 for the [2-3H]glucose and [U-14C]glucose turnover rate; n = 7 for glucose, [6-3H]glucose turnover rate, and [d5]glycerol turnover rate.