TABLE 3

Whole-body and tissue parameters of glucose metabolism during a hyperglycemic clamp in conscious control and knockout mice

ControlKnockout
Whole body
 Glucose infusion rate (μmol · kg−1 · min−1)228.3 ± 9.4100.6 ± 2.8**
 Glucose turnover rate (μmol · kg−1 · min−1)261.1 ± 11.1137.7 ± 7.8**
Hepatic
 Glycogen content (μmol/g)159.8 ± 7.314.4 ± 3.4
 Glycogen synthetic rate (μmol · kg−1 · min−1)57.0 ± 6.410.7 ± 1.6
Muscle
 Glycogen content (μmol/g)17.4 ± 1.812.1 ± 0.6*
 Glycogen synthesis (μmol/g)10.3 ± 1.01.5 ± 0.3
  • Data are means ± SE. [3-3H]glucose was infused for 220 min, and blood glucose concentration was clamped at ∼300 mg/dl for the last 120 min. Male mice ages 7–8 months were fasted for 26 h before infusions.

  • *

    * P < 0.05;

  • P < 0.001 versus control values at each condition (n = 4;

  • **

    ** P < 0.01).