TABLE 4

Summary of flux ratios relative to TCA cycle as determined by analysis of the C2 multiplet areas in the 13C NMR spectrum of MAG

ControlKnockout
Gluconeogenesis (g)2.08 ± 0.510.85 ± 0.04*
Pyruvate recycling (pk + me)1.06 ± 0.200.36 ± 0.18*
Anaplerosis (y)3.15 ± 0.421.21 ± 0.20*
  • Data are means ± SE. In this study, 0.8 ml of D2O was injected intraperitoneally 30 min before continuous infusion of unenriched glycerol and 99% [U-13C3]propionate each at 12 mg/30 g body wt for 60 min. A prime-continuous infusion of [3-3H]glucose was started at 10 min after intraperitoneal injection of D2O and continued for 80 min to determine glucose turnover rate.

  • *

    * P < 0.01 versus control values (n = 4).