In vivo analysis of cell proliferation by immunohistochemistry: Ki-67 labeling indices in mammary glands
| Controls | HMR 1964 | HR 1799 (human insulin; 2 × 20 IU/kg) | |||
|---|---|---|---|---|---|
| 2 × 20 IU/kg | 2 × 50 IU/kg | ||||
| n | 29 | 23 | 14 | 19 | |
| Metestrus | 5.06 ± 2.38 | 5.13 ± 1.69 | 5.10 ± 3.59 | 5.48 ± 3.46 | |
| Diestrus | 6.11 ± 2.39 | 4.74 ± 2.23 | 5.87 ± 2.00 | 6.47 ± 4.04 | |
| Estrus | 6.03 ± 0.73 | 6.06 ± 2.90 | 5.94 ± 3.17 | 5.99 ± 2.83 | |
Data are means ± SD of the indicated number of animals. In vivo proliferation on mammary glands was determined as described in research design and methods. The labeling index indicative for proliferation was expressed as the percentage of positive labeled nuclei per total number of counted nuclei (at least 1,000).