TABLE 3

Primer sequences and PCR conditions for mutation analysis of SLC2A10

PrimerSequence, 5′→3′LocationTanneal (°C)MgCl2 (mmol/l)
G10F1.1*AGG GCA GGA GGG ACA GAGexon 1551
G10R1.1*CAC CCG AGA GGC GGT ATC
G10F2.1GGA TGG TAT GAC AAG GAA CCAexon 2552
G10R2.1CAA GTT GCT CCC GAG GAT G
G10F2.2GGT GGC TTC CTC ATT GAC TGexon 2553
G10R2.2ATC CCC AGG GGG TAC CAG
G10F2.3TGC TCT CCT ATG CCC TCA ACexon 2552
G10R2.3GCT GCC CTG TTA GTT GCT G
G10F2.4CAG GGC ACG CGA TAA CATexon 2552
G10R2.4CAC GGC AAA GCT GAC GAG
G10F2.5TCT GTT GCT AGC TGG CTG TGexon 2552
G10R2.5GGC TGA GGG GTC TCC AGA T
G10F2.6AGC CAA TCT TGT CCA CTG CTexon 2552
G10R2.6ACC CTG CCA GGT CAT CAG
G10F3.1GAA AAT CCC ATT GTT GAG AGGexon 3551
G10R3.1TGC TTT AGA GTA GGG AGC TTG G
G10F4.1TCC ATG CCT GAC CTA GAA CCexon 4552
G10R4.1AAA ATC CTG AAG CTG TGT GC
G10F5.1GAA CCC CAG TGG AAG GTCexon 5552
G10R5.1CAG CAA AAG CAG ACC ACC T
  • *

    * When amplifying exon 1 (using primers G10F1.1 and G10R1.1), 7.5% DMSO was included in the reaction mix.