TABLE 2

Densitometric analysis of Western blots for PKC isoforms

Control groupCAG (0−32 weeks)CALT (16−32 weeks)Diabetic groupDAG (0−32 weeks)DALT (16−32 weeks)
PKC-α1.0 ± 0.11.2 ± 0.11.2 ± 0.11.8 ± 0.2*1.5 ± 0.021.4 ± 0.02
Phosphorylated PKC-α1.0 ± 0.12.1 ± 0.1*0.7 ± 0.11.8 ± 0.3*2.8 ± 0.5*1.0 ± 0.3
PKC-βI1.0 ± 0.10.8 ± 0.10.8 ± 0.11.7 ± 0.1*1.1 ± 0.021.0 ± 0.1
PKC-βII1.0 ± 0.10.7 ± 0.1§0.7 ± 0.1§1.3 ± 0.002§1.0 ± 0.10.9 ± 0.1
PKC-ε1.0 ± 0.11.2 ± 0.041.1 ± 0.11.7 ± 0.06*1.1 ± 0.041.3 ± 0.1
  • Data are means ± SE and are expressed as relative optical density.

  • *

    * P < 0.01,

  • §

    § P < 0.05 vs. control group;

  • P < 0.05,

  • P < 0.01 vs. diabetic group.