TABLE 1

Retinal lactate, pyruvate, L/P ratios, cytosolic and mitochondrial free NADH/NAD+, and lactate production after incubation in 5 or 30 mmol/l glucose at 676 and 36 torr for 2 h

PyruvateLactateLactate/pyruvateCytosolic NADH/NAD+ ×10−3Mitochondrial NADH/NAD+ ×10−3Lactate production
5 mmol/l glucose
    67614.4 ± 2.1278 ± 6019 ± 32.1 ± 0.366 ± 919.3 ± 3.7
    362.0 ± 0.8*526 ± 54*304 ± 12229.8 ± 11.1150 ± 1025.7 ± 4.0
30 mmol/l glucose
    67626.7 ± 2.0§1016 ± 25638 ± 104.1 ± 0.970 ± 17§29.7 ± 4.0*
    362.3 ± 0.8*#1600 ± 254**754 ± 209§#78.8 ± 20.9§#137 ± 26#40.1 ± 2.8**
  • Data are means ± SD, n = 6. Retinal levels of pyruvate and lactate are in pmol/μg DNA; lactate production (sum of lactate in retina + incubation medium) is in nmol · μg DNA−1 · 2 h−1. See research design and methods for calculation of cytosolic and mitochondrial NADH/NAD+. Different from 5 mmol/l glucose at 676 torr:

  • *

    * P < 0.01

  • P ≤ 0.001

  • P < 0.05. Different from 5 mmol/l glucose at 36 torr:

  • §

    § P ≤ 0.001

  • P ≤ 0.03

  • P < 0.005. Different from 30 mmol/l glucose at 676 torr:

  • #

    # P ≤ 0.001

  • **

    ** P < 0.01.