TABLE 2

Retinal metabolite levels and lactate production after incubation in 5 or 30 mmol/l glucose at 676 and 36 torr for 2 h

	Glucose	G6P	F6P	TP	ATP	Lactate production
5 mmol/l glucose
676	301 ± 53	3.58 ± 1.12	0.49 ± 0.33	11.8 ± 2.3	39 ± 3	16.5 ± 2.9
36	323 ± 163	1.84 ± 0.64*	BLD	19.4 ± 6.5†	14 ± 3*	24.5 ± 4.6*
30 mmol/l glucose
676	2398 ± 480*‡	6.72 ± 1.28*‡	1.24 ± 0.36†	16.6 ± 2.5†	64 ± 4*	25.2 ± 1.4*
36	2468 ± 700*‡	3.48 ± 0.40‡§	0.68 ± 0.16‖	30.4 ± 3.3*‡§	46 ± 10‡§	32.5 ± 3.6*‡‖

Data are means ± SD, n = 6. Glucose, glucose 6-phosphate (G6P), fructose 6-phosphate (F6P), triose phosphates (TP), and ATP levels are in pmol/μg DNA. Lactate production (sum of lactate in retina + incubation medium) is in nmol · μg DNA⁻¹ · 2 h⁻¹. BLD, below limits of detection. Different from 5 mmol/l glucose at 676 torr:
*
* P ≤ 0.001
†
† P < 0.01. Different from 5 mmol/l glucose at 36 torr:
‡
‡ P ≤ 0.001. Different from 30 mmol/l glucose at 676 torr:
§
§ P < 0.001
‖
‖ P < 0.004.