TABLE 2

Retinal metabolite levels and lactate production after incubation in 5 or 30 mmol/l glucose at 676 and 36 torr for 2 h

GlucoseG6PF6PTPATPLactate production
5 mmol/l glucose
    676301 ± 533.58 ± 1.120.49 ± 0.3311.8 ± 2.339 ± 316.5 ± 2.9
    36323 ± 1631.84 ± 0.64*BLD19.4 ± 6.514 ± 3*24.5 ± 4.6*
30 mmol/l glucose
    6762398 ± 480*6.72 ± 1.28*1.24 ± 0.3616.6 ± 2.564 ± 4*25.2 ± 1.4*
    362468 ± 700*3.48 ± 0.40§0.68 ± 0.1630.4 ± 3.3*§46 ± 10§32.5 ± 3.6*
  • Data are means ± SD, n = 6. Glucose, glucose 6-phosphate (G6P), fructose 6-phosphate (F6P), triose phosphates (TP), and ATP levels are in pmol/μg DNA. Lactate production (sum of lactate in retina + incubation medium) is in nmol · μg DNA−1 · 2 h−1. BLD, below limits of detection. Different from 5 mmol/l glucose at 676 torr:

  • *

    * P ≤ 0.001

  • P < 0.01. Different from 5 mmol/l glucose at 36 torr:

  • P ≤ 0.001. Different from 30 mmol/l glucose at 676 torr:

  • §

    § P < 0.001

  • P < 0.004.