TABLE 2

Metabolite measurements during hyperinsulinemic-euglycemic clamp

ParameterCon-GlyCon-LipMet-Lip
Insulin (mU/l)447 ± 53497 ± 56452 ± 19
Glucose (mmol/l)8.04 ± 0.258.26 ± 0.128.44 ± 0.18
FFAs (mmol/l)0.23 ± 0.032.82 ± 0.45*2.92 ± 0.45*
TAGs (mmol/l)0.39 ± 0.051.21 ± 0.14†1.32 ± 0.12†
Lactate (mmol/l)1.58 ± 0.081.46 ± 0.122.22 ± 0.27
Liver glycogen (nmol/mg)89 ± 1384 ± 17159 ± 31*‡
RQ glycogen (nmol/mg)58 ± 854 ± 461 ± 4
Liver triacylglycerol (μmol/g)22.2 ± 2.333.3 ± 3.3*34.3 ± 2.9*
  • Data are means ± SE. Metabolite concentrations after 5 h lipid (Lip) or glycerol (Gly) infusion plus 2 h clamp in rats pre-administered with metformin (Met) or vehicle (Con). By ANOVA, plasma FFAs (P < 0.001) plasma TAG (P < 0.001), liver glycogen (P < 0.05), and liver triacylglycerol (P < 0.05) differed between groups. Post hoc: *P < 0.05 and †P < 0.001 vs. Con-Gly; ‡P < 0.05 vs. Con-Lip. n = 6–8 per group. RQ, red quadriceps.