TABLE 1

Effect of MTII administration on body weight and food intake

Initial weight (g)Final weight (g)Weight loss (g)Cumulative food intake (g)Cumulative food intake (kcal)
Short-term studies (24-h)
 C57BI/6J lean and obese
  PBS/chow32.20 ± 0.8932.33 ± 1.090.30 ± 0.214.09 ± 0.3014.30 ± 1.00
  MTII/chow32.10 ± 0.7630.33 ± 0.751.42 ± 0.232.55 ± 0.218.89 ± 0.72§
  Pair-fed to MTII/chow32.06 ± 0.6130.18 ± 0.561.57 ± 0.322.55 ± 0.038.89 ± 0.09§
  PBS/high-fat44.07 ± 0.4242.51 ± 0.721.43 ± 0.23‖‖2.83 ± 0.312.83 ± 1.36
  MTII/high-fat43.81 ± 0.7641.30 ± 0.712.51 ± 0.21‡‡1.15 ± 0.22§‡‡5.22 ± 1.00§††
  Pair-fed to MTII/high-fat44.27 ± 1.0842.20 ± 0.942.07 ± 0.191.15 ± 0.01§5.22 ± 0.03§
Ob/ob
  PBS69.74 ± 10.569.38 ± 1.220.36 ± 0.289.7 ± 3.233.76 ± 11.14
  MTII69.70 ± 2.0067.45 ± 1.822.26 ± 0.246.4 ± 0.622.27 ± 2.09
 UCP1-DTA
  PBS40.04 ± 2.636.39 ± 2.63.65 ± 0.223.09 ± 0.4310.75 ± 1.50
  MTII39.27 ± 2.234.48 ± 2.14.79 ± 0.22#1.78 ± 0.426.19 ± 1.46
  Pair-fed39.55 ± 2.034.01 ± 2.05.54 ± 0.22§1.79 ± 0.016.19 ± 0.03
Long-term studies
 C57BI/6J 4-day lean and obese
  PBS/chow30.42 ± 0.8229.60 ± 0.690.82 ± 0.3616.07 ± 0.9355.91 ± 3.22
  MTII/chow30.43 ± 0.5828.07 ± 0.572.37 ± 0.2212.95 ± 0.6445.06 ± 2.22
  PBS/high-fat40.50 ± 1.39##38.14 ± 1.20##2.36 ± 0.2711.48 ± 0.54¶¶52.64 ± 2.87
  MTII/high-fat40.63 ± 1.58##35.07 ± 1.25##5.57 ± 0.38§§7.45 ± 0.53§§33.75 ± 2.42‡‡
 DIO-C57BI/6J 8-day MTII + leptin  vs. MTII treatment
  PBS34.62 ± 0.9332.63 ± 0.991.99 ± 0.4025.01 ± 0.74118.30 ± 3.49
  MTII34.58 ± 1.3029.28 ± 1.055.3 ± 0.3617.71 ± 0.7083.76 ± 3.32
  MTII + leptin34.33 ± 1.4627.94 ± 1.146.39 ± 0.45**17.40 ± 0.5782.28 ± 2.69
  Pair-fed33.59 ± 0.9429.32 ± 0.584.27 ± 0.43§17.30 ± 0.0182.25 ± 0.04
  • Data are means ± SE. Short-term studies: Effect of 24 h of MTII administration on body weight and cumulative food intake in lean C57BI/6J, DIO, ob/ob, and UCP1-DTA mice. Long-term studies: effects of 4 days of MTII administration in lean C57BI/6J and DIO mice as well as 8 days of either MTII or MTII plus leptin administration in DIO mice on body weight and cumulative food intake.

  • P < 0.05,

  • P < 0.01,

  • §

    § P < 0.001,

  • P < 0.0001 vs. the respective PBS-treated group;

  • P < 0.05,

  • #

    # P < 0.01,

  • **

    ** P < 0.001 vs. the pair-fed group;

  • ††

    †† P < 0.05,

  • ‡‡

    ‡‡ P < 0.01,

  • §§

    §§ P < 0.001 vs. the MTII-treated group on chow;

  • ‖‖

    ‖‖ P < 0.01,

  • ¶¶

    ¶¶ P < 0.0001 vs. the PBS-treated group on chow;

  • ##

    ## P < 0.01 vs. both groups on chow.