TABLE 2

mRNA levels of positively or negatively adrenergic-regulated genes in BAT of cold-exposed mice

MouseHours at 4°CACCUCP-1GMPrPGC-1α
WT01.0 ± 0.11.0 ± 0.11.0 ± 0.31.0 ± 0.1
60.5 ± 0.22.4 ± 0.213.2 ± 2.21.2 ± 0.1
241.1 ± 0.22.4 ± 0.116.5 ± 1.53.9 ± 0.6
Dio2−/−01.0 ± 0.30.8 ± 0.11.2 ± 0.30.9 ± 0.2
60.3 ± 0.23.8 ± 0.4*17.9 ± 1.8*3.4 ± 0.5*
240.5 ± 0.2*5.2 ± 0.6*18.2 ± 2.92.9 ± 0.3
Hypothyroid01.0 ± 0.30.6 ± 0.2*NDND
60.3 ± 0.1*1.2 ± 0.3*NDND
  • Data are means ± SD of four animals. ACC mRNA levels were determined by RT-PCR. UCP-1, GMPr, and PGC-1α mRNA levels were determined by Northern blot, and the bands of interest were cut from the RNA-containing filter and counted in a liquid scintillation counter. The signal in each lane was corrected for RNA loading by the intensity of the 28S ribosomal RNA band.

  • *

    * P < 0.05 vs. the same time in WT animals by ANOVA. Hypothyroid mice are WT. This experiment was performed twice.