Relative quantitation of mutant and wild-type mRNA transcript levels.
| Control 2 | R171X | I414G415ATCG→CCA | P291fsinsC | G207D | R229P | |
|---|---|---|---|---|---|---|
| Median ΔCt | 0.145 | 1.095 | 18.01 | 3.845 | −0.105 | 0.745 |
| Interquartile range | 0–0.46 | 0.37–1.81 | 6.47–20.13 | 0.19–12.08 | 0.81–1.09 | −0.22 to 26.62 |
| ΔΔCt | 0.36 | 1.305 | 18.22 | 4.055 | 0.105 | 0.955 |
| % Wild type | 78 | 40 | <0.01 | 6 | 93 | 52 |
| P value | 0.156 | 0.009 | <0.0001 | 0.001 | 0.546 | 0.03 |
ΔCt refers to the difference between the crossing point for the C allele and the T allele. ΔΔCt refers to this figure normalized to the controls. The median ΔCt values and interquartile range are shown for control 2, R171X, 1414G415ATCG→CCA, P291fsinsC, R229P, and G207D. The calculated levels of the mutant transcripts are shown with their P values as determined by Mann-Whitney test.